AviSYBR™

AviSYBR™
Power Your RT-PCR with SYBR Green Master Mix

Unveiling SYBR Green:
The Heart of Real-Time PCR

SYBR Green is a fluorescent dye that revolutionized DNA analysis. Its mechanism is elegantly simple yet incredibly powerful. When SYBR Green molecules come into contact with double-stranded DNA (the product of PCR), they bind to the minor groove of the DNA helix. This binding event triggers a dramatic increase in the dye's fluorescence. As your PCR reaction proceeds, more and more DNA is amplified, leading to a corresponding increase in fluorescence.

This real-time fluorescence monitoring is the key to SYBR Green's success. It allows you to track the amplification of your target DNA in real-time, eliminating the need for post-PCR analysis. It's worth noting that there are different types of SYBR Green dyes, each with slight variations in their properties. AviSYBR™ is optimized to provide maximum sensitivity and specificity for your RT-PCR experiments.

Why Choose a SYBR Green Master Mix?

A master mix streamlines your PCR workflow. It's a pre-mixed solution containing all the essential components for PCR: SYBR Green dye, Taq polymerase (the enzyme that copies DNA), dNTPs (building blocks for new DNA), MgCl2 (essential for polymerase activity), and optimized buffer. This eliminates the need for multiple pipetting steps, reducing the risk of errors and contamination.

Moreover, the consistent composition of a master mix ensures reproducibility across experiments. The carefully balanced formulation is designed to maximize the sensitivity of your PCR, enabling the detection of even low amounts of target DNA.

Advantages of AviSYBR™ 

AviSYBR™ takes your RT-PCR to the next level. Its key advantages include:

• Dual Hot-Start Taq Polymerase: This unique enzyme blend combines chemically modified and antibody-mediated hot-start mechanisms, ensuring high specificity by preventing non-specific amplification at lower temperatures.
• Enhanced Sensitivity: AviSYBR™ is formulated with a proprietary buffer system that enhances the fluorescence signal of SYBR Green, allowing you to detect subtle changes in gene expression.
• User-Friendly: Simply add your template DNA, primers, and water to the AviSYBR™ master mix. No need to worry about optimizing individual components.
• Versatility: Whether you're studying gene expression, genotyping, or microbial detection, AviSYBR™ is adaptable to a wide range of RT-PCR applications.

How AviSYBR™ Empowers Your PCR

1. Target Amplification: As your PCR cycles proceed, your target DNA is copied exponentially.
2. SYBR Green Binding: The SYBR Green dye in AviSYBR™ binds to the newly formed double-stranded DNA.
3. Fluorescence Detection: This binding triggers a fluorescent signal, which your RT-PCR instrument measures.
4. Real-Time Quantification: The increasing fluorescence directly correlates to the amount of your target DNA, enabling precise quantification throughout the PCR process.

The SYBR Green Advantage: A Comparison

SYBR Green offers a cost-effective and flexible alternative to probe-based methods like TaqMan and molecular beacons. It's ideal for scenarios where cost and simplicity are priorities.

AviSYBR™:
Your Trusted Partner in RT-PCR Success

Whether you're a seasoned researcher or new to RT-PCR, AviSYBR™ is designed to simplify your workflow and elevate your results. Its proven performance, user-friendly format, and competitive pricing make it the perfect choice for research, diagnostics, and educational settings.

Ready to experience the power of AviSYBR™?
Contact us today for a free sample and discover how it can transform your RT-PCR.