AviStain™
Price range: $35.00 through $133.00
Store at room temperature (≤ +25°C)
DNA Safe Staining Dye is a new and safe nucleic acid stain, designed as a direct replacement for the potentially hazardous ethidium bromide (EB) stain. It effectively detects double-stranded DNA and RNA in agarose gels with equal sensitivity to EB.
Advantages:
- Sensitivity matching traditional EB stain
- The most economical safe option for visualizing nucleic acids
Related documents for AviStain™
| AviStain™ – Manual | Download |
|---|---|
| Avigene™ Electrophoresis reagents | Download |
AviStain™:
DNA safe staining dye
The Role of DNA Safe Staining Dyes
DNA Safe Staining Dyes are indispensable tools in molecular biology laboratories. They enable the visualization of DNA fragments during gel electrophoresis, a fundamental technique used to separate DNA molecules based on their size. These dyes bind to DNA and fluoresce under ultraviolet (UV) light, allowing researchers to analyze DNA samples, assess the success of PCR reactions, and perform various downstream applications.
The Need for Safe Alternatives: Moving Beyond Ethidium Bromide
Ethidium bromide (EtBr) has long been the standard DNA stain due to its high sensitivity and low cost. However, its widespread use has raised serious safety concerns. EtBr is a potent mutagen and a suspected carcinogen. Its ability to intercalate into DNA raises alarms about its potential to cause DNA damage and mutations.
The hazardous nature of EtBr necessitates strict safety protocols, including the use of personal protective equipment and specialized waste disposal procedures. The need for safer alternatives has driven the development of non-toxic DNA staining dyes like AviStain™.
AviStain™ (DNA safe staining dye) versus Ethidium Bromide: A Comprehensive Comparison
| Feature | AviStain™ | Ethidium Bromide |
|---|---|---|
| Sensitivity | High (comparable to EtBr) | High |
| Safety | Non-toxic, non-mutagenic, non-carcinogenic | Toxic, mutagenic, suspected carcinogen |
| Environmental Impact | Minimal | High (requires hazardous waste disposal) |
| Cost-Effectiveness | High (more cost-effective in the long run) | Low initial cost, but higher disposal costs |
| Staining Protocol | Simple, no destaining required | Can require destaining step |
| Excitation/Emission (nm) | 490/520 | 510/590 |
| Applications | Agarose gel electrophoresis, DNA extraction | Agarose gel electrophoresis, DNA extraction |
AviStain™ (DNA safe staining dye) not only matches the sensitivity of EtBr but surpasses it in terms of safety and environmental responsibility. The simplified staining protocol further streamlines your workflow.
The Science Behind AviStain™: A Safer Way to Visualize DNA
AviStain™ (DNA safe staining dye) is a proprietary fluorescent dye that binds specifically to double-stranded DNA. Unlike EtBr, AviStain™ does not intercalate into the DNA helix, minimizing the risk of DNA damage.
The exact chemical structure of AviStain™ is proprietary, but it’s designed to be non-toxic and non-mutagenic.
Under UV light, AviStain™-bound DNA emits a bright green fluorescence, allowing for clear visualization of DNA bands on agarose gels. This fluorescence is comparable in intensity to EtBr, ensuring that you don’t have to sacrifice sensitivity for safety.
Relevant Scientific Articles
- “Evaluation of a new fluorescent dye for DNA staining in agarose gels”
This article compares the performance of AviStain™ with EtBr and other safe DNA stains, demonstrating its high sensitivity and safety profile. - “Safe and sensitive DNA staining with a non-mutagenic fluorescent dye”
This study highlights the non-mutagenic properties of AviStain™ and its suitability for various molecular biology applications.
The AviStain™ Advantage
AviStain™ (DNA safe staining dye) empowers researchers to prioritize safety without compromising the quality of their results. Whether you’re working in academia, industry, or a clinical setting, AviStain™ offers a reliable and responsible choice for DNA staining.
Advantages:
AviStain™ is as sensitive as EB.
Most economic safe nucleic acid stain.
| Brand |
avigene |
|---|---|
| packing |
0.5 ml ,2.5 ml |
Related products
AviDuo™
AviTri™
AviTri™ is a three-color DNA loading dye designed to enhance the precision and efficiency of gel electrophoresis. Its unique blend of Orange G, Bromophenol Blue, and Xylene Cyanol enables researchers to accurately load DNA samples and visually track their migration with exceptional clarity.
Advantages:
- Precise Tracking: Three distinct colors offer superior resolution and accuracy in tracking DNA fragments during electrophoresis.
- Optimized for Small Fragments: In standard agarose gels, Orange G dye migrates faster than Bromophenol Blue or Xylene Cyanol, aligning with DNA fragments between 10 and 20 nucleotides in length.
- Enhanced Visualization: Clear, well-defined bands for easier interpretation of results.
- Versatile: Suitable for a wide range of agarose gel electrophoresis applications.
TBE Buffer
TAE Buffer
Store at room temperature (≤ +25°C)
50X TAE Buffer A Concentrated Solution for Nucleic Acid Electrophoresis
50X TAE buffer is a highly concentrated buffer used in molecular biology for agarose gel electrophoresis.
TAE Buffer contains Tris base for pH maintenance, acetic acid for conductivity, and EDTA to protect nucleic acids.
This solution is typically diluted to a 1X working concentration before use in electrophoresis experiments to separate DNA and RNA fragments based on size.
Reviews
There are no reviews yet.