AviAHot™

Keep in freezer (≥ -20°C)

Apta Hot Start Taq DNA Polymerase

AviAHot ™ , Apta Hot Start Taq DNA Polymerase is a mixture of  Taq DNA polymerase and a temperature sensitive aptamer-based inhibitor.

The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions.

The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme.

AviAHot™ (Apta Hot Start Taq DNA Polymerase) is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.

The activated AviAHot™ maintains the same functionality as Taq DNA polymerase:

It catalyzes 5′ → 3′ synthesis of DNA, has no detectable 3′ → 5′ proofreading exonuclease activity.