Molecular Biology Enzymes & Reagents
AviAHot™
Keep in freezer (≥ -20°C)
Apta Hot Start Taq DNA Polymerase
AviAHot ™ , Apta Hot Start Taq DNA Polymerase is a mixture of Taq DNA polymerase and a temperature sensitive aptamer-based inhibitor.
The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40 °C, but releases the enzyme during normal PCR cycling conditions.
The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme.
AviAHot™ (Apta Hot Start Taq DNA Polymerase) is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.
The activated AviAHot™ maintains the same functionality as Taq DNA polymerase:
It catalyzes 5' → 3' synthesis of DNA, has no detectable 3' → 5' proofreading exonuclease activity.
AviFix™
Store at room temperature (≤ +25°C)
RNA Fixation - Prevents RNA degradation
AviFix™ is an aqueous, non toxic, tissue and cells storage solution intended for the preservation of RNA for later isolation. AviFix™ is a preservation solution that allows recovery of intact RNA from tissues and cell culture.
Samples in AviFix™ solution can be stored indefinitely at -20 °C with no RNA degradation.
AviFix™ solution can be used for the storage of tissues, cells, bacteria and yeasts as RNA Fixation.
AviLong™
Keep in freezer (≥ -20°C)
Pfu DNA Polymerase
Ideal for Long PCR with Minimal Errors
AviLong™ is a chimeric Pfu polymerase that contains a DNA binding protein at the N-terminal portion of the gene.
AviLong™ maintains significant activity even after exposure to 99 °C or repeated exposure to 98 °C, exhibiting higher processivity and extension rates compared to Pfu DNA polymerase.
It is particularly suitable for PCR and primer extension reactions that require high fidelity, especially when amplifying PCR fragments larger than 3 kb."
AviPfu™
Keep in freezer (≥ -20°C)
AviPfu™
An essential tool in molecular biology applications, is synonymous with precision and reliability.
AviPfu™ proudly presents a recombinant and exquisitely purified protein form of Pfu DNA Polymerase, setting a new standard in enzymatic performance.
Renowned for its distinctive 3´ > 5´ proofreading activity, AviPfu™ surpasses traditional Taq DNA Polymerases by delivering over 10-fold higher PCR fidelity.
This exceptional fidelity ensures accurate DNA synthesis and replication, crucial for demanding research tasks like sequencing, cloning, and mutagenesis.
Researchers trust AviPfu™ for its unwavering commitment to quality and excellence, promoting error-free amplification of DNA fragments and minimizing the risk of introduced mutations.
Elevate your molecular biology experiments with AviPfu™, the pinnacle of enzymatic precision and fidelity in the scientific community."
AviTaq™
Keep in freezer (≥ -20°C)
Taq DNA Polymerase.
AviTaq™ is a chromatography highly purified enzyme with an optimized buffer to give you a specific band.
AviTaq™ (Taq DNA Polymerase) is provided with an exclusive 10 X reaction buffer to improve sub-optimal PCR caused by templates, high degree of secondary structure or GC-rich regions.
AviURT™
Keep in freezer (≥ -20°C)
Thermo-resistant H-minus MMLV - optimum activity at 55 °C
AviURT™, MMLV reverse transcriptase, is a genetically modified RNA-dependent DNA polymerase that necessitates a DNA primer and an RNA template to synthesize a complementary DNA strand.
AviURT™ (MMLV Reverse Transcriptase) exhibits no RNase H activity, thereby preventing RNA degradation during the synthesis of the first cDNA strand. This characteristic leads to enhanced yields of full-length cDNA from lengthy templates in comparison to other reverse transcriptases.
AviURT™ (MMLV Reverse Transcriptase) retains its functionality across a broad temperature range (50-60°C), rendering it an optimal choice for reverse transcription of RNAs with complex secondary structures.
AviCHot™
Keep in freezer (≥ -20°C)
Chemical Hot Start Taq DNA Polymerase
AviCHot™, Chemical Hot Start Taq DNA Polymerase, is DNA polymerase is a chemically modified Taq DNA polymerase.
The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 60°C, but releases the enzyme during normal PCR cycling conditions.
The chemically-modified hot start mechanism requires a high temperature incubation step to activate the enzyme.
The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.
The activated enzyme maintains the same functionality as Taq DNA polymerase:
It catalyzes 5' → 3' synthesis of DNA, has no detectable 3' → 5' proofreading exonuclease activity.
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This product has multiple variants. The options may be chosen on the product page