Molecular Biology Enzymes & Reagents

Keep in freezer (≥ -20°C) Chemical Hot Start Taq DNA Polymerase AviCHot™, Chemical Hot Start Taq DNA Polymerase, is DNA polymerase is a chemically modified Taq DNA polymerase. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 60°C, but releases the enzyme during normal PCR cycling conditions. The chemically-modified hot start mechanism requires a high temperature incubation step to activate the enzyme. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification. The activated enzyme maintains the same functionality as Taq DNA polymerase: It catalyzes 5' → 3' synthesis of DNA, has no detectable 3' → 5' proofreading exonuclease activity.